Views: 23 Author: Site Editor Publish Time: 2024-03-08 Origin: Site
The main component of yeast beta glucan is composed of glucan molecules, which is a polysaccharide compound. It mainly contains glucose molecules connected by β-1,3-glucan bonds and β-1,6-glucan bonds.
Regarding yeast beta glucan content, general specifications vary by manufacturer and product. Generally, the yeast glucan content of a product will be detailed on the product label. Typical conventional specifications may be between 70 and 90 percent.
Nanjing Tessinn Biotechnology Co., Ltd. can accept customized yeast beta glucan content, but the specific customizable range requires detailed communication and negotiation.
Detecting the effective content of yeast glucan usually requires the use of some specific experimental methods and instruments. Instruments that may be used:
●High performance liquid chromatography (HPLC): Used to separate the components in a mixture and determine the concentration of each component. HPLC is an efficient and accurate analytical technique commonly used to determine yeast glucan content.
●UV-Vis Spectrophotometer: Used to measure the absorbance at a specific wavelength in a solution. This can be used for quantitative analysis of yeast glucans.
High-performance liquid chromatography (HPLC) is a commonly used method to detect yeast glucan content. This method achieves high accuracy and sensitivity by isolating yeast glucans in samples and measuring their concentrations.
High-Performance Liquid Chromatography (HPLC) is an efficient and highly sensitive technology for separating and analyzing compounds. It is a liquid chromatography technology widely used in chemistry, biochemistry, pharmacy, environmental science and other fields.
working principle:
HPLC separates a sample in a liquid phase and then uses a detector to measure the concentration of each component. This process usually includes a high-pressure pump, a sample injection system, a chromatographic column, a detector and data recording system.
●High-pressure pump: Push solvent through the column at high pressure, ensuring that samples are separated quickly and efficiently.
●Sample injection system: Inject the sample to be measured into the high-pressure mobile phase in a small volume, allowing it to enter the chromatographic column.
●Chromatography Column: Within a column, compounds are separated based on their properties through interaction with the column packing. Filler selection can be adjusted based on analytical needs.
●Detector: The detector measures the concentration of each component in the solution. The most common one is the UV-Vis Detector, but fluorescence detectors, conductivity detectors, etc. can also be used.
●Data recording system: records and processes the data output by the detector to generate analysis results.
Application areas:
HPLC is widely used in biochemistry, pharmaceuticals, environmental monitoring, food analysis and other fields. It is used to analyze and determine compounds in samples, such as drugs, proteins, nucleic acids, organic matter, etc.
Advantage:
High resolution: Ability to effectively separate components in complex mixtures.
High sensitivity: Able to detect compounds at low concentrations.
Automation: Large-scale sample analysis can be performed to improve work efficiency.
Accuracy and Repeatability: Provides accurate analysis results with high repeatability.
When testing yeast glucan, it is usually necessary to use a specific type of sugar column (Column for Sugar Analysis) as an accessory to a high-performance liquid chromatograph (HPLC). The choice of sugar column in a chromatography column is very important for efficient separation and analysis of yeast glucans.
Common types of sugar columns used for sugar analysis include the following:
●Ion Exchange Column: Used to separate sugar molecules based on their charged properties. Ion exchange columns can selectively separate sugar molecules with different charges.
●Gel Filtration Column: Separates sugar molecules based on their size. Larger sugar molecules move more slowly through the column, allowing the sugars to be separated.
●Affinity Chromatography Column: Use specific affinity chromatography media, such as affinity resin, to selectively separate based on the specific interaction between yeast glucan and the media.
●Carbohydrate Column: A column specially designed for carbohydrate analysis, usually using silicone packing. This column provides good separation of polysaccharides such as yeast glucan.
